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DNP derivatives of the standard aldehydes were prepared according to the commonly used method described by Esterbauer et al. [ 17].
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Dehydrogenase activity was determined using method described by Weddle and Jenkins (1971).
In this case, vermiwash was produced using method described by Pramanik (2010) with slight modification.
Biofilm growth was quantified using method described by Kaplan et al. [33].
For all strains genomic DNA was prepared using method described by Marmur [ 15].
*Standard errors adjusted for clustering on two population averaged models, using method described by Miglioretti and Heagerty.
The 95% confidential intervals were calculated for fitted parameters using method described by Motulsky (see Supplementary Materials online).
Iodine was assayed in spot urine samples using method described by J.Dunn et al. (1993) after wet digestion with ammonium persulfate.
For each indicator, statistical differences between sexes were determined by approximate 95% confidence intervals (95% CI), which were calculated using method described by Jagger et al. [ 26].
Transthoracic echocardiogram was performed within the first 3 days of admission to assess left ventricular ejection fraction using method described by Simpson.
The data were collected using methods described by [45].
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