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Our analysis included additional outgroup sequences, and used maximum likelihood reconstruction methods (as opposed to parsimony).
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This can cause so-called truncation artefacts, which can at least partially be corrected with PET emission data using maximum likelihood reconstruction of attenuation and activity (MLAA) [18].
Phylogenetic analyses were conducted on all sequence data sets (individual and combined) using maximum likelihood reconstruction and Bayesian inference.
With this aim, we designed a flowchart of bioinformatic analyses to confirm and standardize the phylogenetic signal of each family using maximum likelihood reconstruction (AMLR) before to its characterization by sequence logos and HMMs.
Phylogenetic analyses were conducted on sequence datasets from individual and combined DNA regions using maximum likelihood reconstruction with PhyML [ 66] and Bayesian inference as implemented in MrBayes 3.1.2 [ 67].
First, we looked at species divergence in the PC axes identified above by reconstructing the ancestral states for each protein across the phylogeny on each axis in the PCA using maximum likelihood reconstruction in the ace function of the ape package in R [ 42].
Nonetheless, group VI was consistent when different methods for phylogenetic reconstruction were used (maximum likelihood and Bayes data are available on request).
We have used Maximum Likelihood (ML) and Bayesian methods of phylogeny reconstruction together with the stripping of the most rapidly evolving sites in the dataset.
Estimates used maximum likelihood.
The tree of the CYP71C subfamily in Poaceae was generated on the phylogeny.fr website [ 34] using maximum likelihood as reconstruction method (PhyML program).
Because Mk2 models cannot account for the effect of the character on rates of speciation and extinction [ 14, 37, 78], we complemented our ancestral character reconstructions by obtaining marginal estimates of ancestral node states over 1,000 Beast trees under our best fitting BiSSE model using maximum likelihood (ML) reconstructions (see [ 79] for a similar approach).
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