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As HIV FISH probe, the pNL4-3 plAdachi(Adachi et al. 1986) was used, isolated from E.coli (Plasmid isolation kit, Macherey-Nagel, Düren, Germany).
For each transformation experiment a plasmid quantity of 400 ng was used (isolated from either the relevant B. longum strain or E. coli EC101) employing electroporation of B. longum subsp. longum NCIMB 8809.
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We used isolates from two samples of children form two different years.
A second phylogenetic analysis was undertaken that used isolates from this study and all published full-length WNV sequences from North America available on GenBank.
Some models use isolated cardiomyocytes from preclinical species, having an impact on animal use.
Most of these studies were conducted in-vitro using isolated uteri from rodents.
This approach was tested by using isolates from known sources (cattle, sheep, and chickens), which allowed us to compare our predictions with the true origin of each strain.
Part of their study was performed using isolates from 6 outbreaks.
We have analyzed the differences in patient age distribution between influenza A subtypes, using isolates from the 2006 2007 and 2007 2008 influenza seasons in New York State.
We compared 2 settings using isolates from non-human samples.
Data using isolates from antimony-responsive patients are shown in Figure 2.
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