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After each modification, approximately one dozen colonies were evaluated, and the best-growing colony was used in subsequent work.
SDS at 1% was used in subsequent work.
Authors' response: This simplification is noted by Wagner [ 15]: "For reasons of computational simplicity... ", and, as the reviewer notes, has been widely used in subsequent work (e.g. Siegal and Bergman 2002 [ 18]).
Although two concentrations of Cel7A MAb (10 and 50 μg/ml diluted in 1x Phosphate-Buffered Saline or PBS) were initially assessed, as both concentrations gave similar absorbance values, a MAb concentration of 10 μg/ml was used in subsequent work.
Similar(56)
In this paper, the adopted datasets, involving targets of ENZYME, ION CHANNEL, GPCR, and NUCLEAR RECEPTOR, were originally from [ 13] and further used in subsequent works [ 8– 10].
The variations observed could be used selected for and used in subsequent breeding work.
Findings from this study could be used in subsequent fine-mapping work and applied to marker-assisted selection of dairy production and health traits.
The biological processes and test methods were organized into a matrix that served as a working document used in subsequent steps [this working document (BCCP 2010) is available online: http://coeh.berkeley.edu/greenchemistry/cbcrpdocs/matrix.pdf].
The treatments were used in subsequent assays.
This concentration was used in subsequent reactions.
Transformant JP7.7 was used in subsequent experiments.
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