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A total of seven columns were used in parallel experiments.
A control virus lacking a shRNA insert was used in parallel experiments as control.
In order to achieve maximal experimental uniformity, transplanted EPC and EPC-CM were derived from the same donors and used in parallel experiments.
Membrane strips used in parallel experiments were exposed on a same film.
In order to achieve maximal experimental uniformity, transplanted EPCs were derived from the same donors and used in parallel experiments.
An isotype control (IgG1κ; BioLegend, San Diego, CA, USA) antibody was used in parallel experiments at the corresponding concentrations.
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Clinically established antibiotic, cefepime was used in parallel experiment as a positive drug control.
These physical measurements will be used to characterize the beam used in parallel biological experiments.
Nonetheless, to make sure that the observed behavior was not due to some non-specific effect of the carrier, or to the presence of extra enhancers, GFP vectors bearing just the basal promoter (EpGFP) were used in parallel control experiments.
Equal fractions of each total cellular lysate were also used in parallel immunoblot experiments to detect total VSV N (middle panel) or actin protein levels.
Moreover, for additional control experiments, small HSPs denatured by heat (100°C, 20 min) were used in parallel sets of experiments.
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