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The results are presented in Figure 2. The samples are taken from two animals not used in microarray study.
The first group consists of standard methods which have been conventionally used in microarray study and do not consider reference datasets.
In order to confirm the statistical significance of our findings, we performed quantitative real-time PCR (qPCR) analysis of the relevant genes in our original samples used in microarray study.
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Pair-wise comparisons between conditions/groups/treatments are frequently used in microarray studies.
The clinical features of the 22 CNS GCT cases used in microarray studies are listed in Additional file 1-B in order to help correlation with the results of the genomic analysis.
For instance, this approach can be used in microarray studies to accurately identify the most important subset of polymorphisms even when the number of variables greatly exceeds the number of participants.
Total RNA from brain abscesses of MyD88 KO and WT mice was isolated using TRIzol® reagent (Invitrogen) and subjected to DNAse treatment prior to use in microarray studies.
Environmental ozone can rapidly degrade cyanine 5 (Cy5), a fluorescent dye commonly used in microarray gene expression studies.
Shown is the demography of the female study population used in the microarray study.
Isolation of total RNA for mosquitoes used in the microarray study followed the protocol recommended by Affymetrix Inc. (Santa Clara, CA).
Loop design and dye swap were used in the microarray study.
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