Sentence examples for used in microarray gene from inspiring English sources

Environmental ozone can rapidly degrade cyanine 5 (Cy5), a fluorescent dye commonly used in microarray gene expression studies.

The solid-phase approach relies on probes immobilized onto an array surface [ 123], like those used in microarray gene expression studies.

For differential expression analysis, we applied two complementary methods, both established and commonly used in microarray gene expression analysis: a hierarchical analysis of variance (ANOVA) (false discovery rate (FDR) <10%) and an empirical Bayes moderated single-group t test per gene (FDR <10%).

Although we do not use Unigene annotations of probes to identify the targeted genes (see discussion section for explanation) this allowed us to associate Entrez Gene ids with EST accession numbers, which are commonly used in microarray genes lists.

Due to its high sensitivity and specificity, fluorescence analysis has been extensively used in microarray technology [ 1], gene expression monitoring [ 2], protein diffusion [ 3] or in vivo chemical elements uptake and localization studies [ 4].

Affymetrix GeneChip Porcine Genome Array, which contains 24,123 probe sets to interrogate 23,256 transcripts in pig, represents 20,201 genes, was used in microarray analysis.

We verified changes in microarray gene expression using quantitative real-time PCR (QRT-PCR) for representative genes from our analysis.

The use of p-value cutoffs alone in microarray gene expression studies leads to the potential underestimation of variance, which can result in a large number of false positive differentially expressed genes.

To validate the microarray results, expression of 18 of the 933 differentially expressed genes was subjected to qPCR using the same RNA samples as those used in the microarray, and gene specific primer sets (Tables 1, 2).

The oligo-dT based amplification method used in 3' gene expression microarray has a strong position effect rendering a signal bias towards the probes targeting the 3' ends of genes.

We established a cell line that constitutively expresses a dominant-negative LEF-1 and it was used in a gene expression microarray analysis.