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RF-based variable importance measures were previously used in microarray expression data analyses to select a relatively small set of informative genes for disease/sample classification [ 14, 15].
Two-tailed student's t-test was used in microarray expression analysis and Fisher's Z transformation was used to adjust p value.
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For differential expression analysis, we applied two complementary methods, both established and commonly used in microarray gene expression analysis: a hierarchical analysis of variance (ANOVA) (false discovery rate (FDR) <10%) and an empirical Bayes moderated single-group t test per gene (FDR <10%).
Environmental ozone can rapidly degrade cyanine 5 (Cy5), a fluorescent dye commonly used in microarray gene expression studies.
The solid-phase approach relies on probes immobilized onto an array surface [ 123], like those used in microarray gene expression studies.
Since pre-amplification was not used in microarray assays, miRNA expression levels in microarray were compared to those in miRNA TaqMan Array A without pre-amplification; 84 miRNAs were detected in both microarray and qPCR-array (Ct ≤ 35).
Due to its high sensitivity and specificity, fluorescence analysis has been extensively used in microarray technology [ 1], gene expression monitoring [ 2], protein diffusion [ 3] or in vivo chemical elements uptake and localization studies [ 4].
The first data set was derived from FAC-sorting GFP-positive and -negative cells from the subpallium and pallium of E14.5 Dlx5/6 Cre-IRES-eGFP embryos, which were then used in microarray analyses to compare the expression profiles between the different populations of sorted cells (42).
Furthermore, the gene IDs can be used for analysis in microarray expression databases to determine whether these are coregulated.
Comparative expression analysis is often used in microarray-based analyses of expression changes due to systematic perturbation to find overlapping effects on biological pathways.
For technical validation of differentially expressed genes, we performed semi-quantitative RT-PCR using RNA extracted from additional samples that were collected in each brain region from tissue adjacent to that used in the microarray expression study.
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