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Extracted RNA was then used in microarray experiments using Illumina Sentrix HT-12_v3_BeadChip arrays (Illumina, San Diego, California).
Ca. Pelagibacter ubique cells used in microarray experiments were grown in batch cultures as described above.
HTCC1062 cells used in microarray experiments were grown in batch cultures (50 ml each in 250 ml polycarbonate flasks).
However, a weakness of this work was the small number of samples used in microarray experiments.
This is particularly relevant for the test cohort used in microarray experiments.
The experiments were performed on the same RNA samples used in microarray experiments.
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Equal amount of RNA samples from all the four biological replicates used in microarray experiment were pooled together for use in RT-PCR.
Equal amount of RNA samples from all the four biological replicates used in microarray experiment were pooled together for electrophoretic analysis.
Reverse transcription was performed with 500 ng total RNA (same as used in microarray experiments) using the iScript cDNA synthesis kit (Bio-Rad).
Fluorescently labeled tmRNA was then used in microarray hybridization experiments.
Both single-stranded and double-stranded nucleic acid have been used successfully in microarray experiments.
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