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The genomes of all sequenced strains can be used in microarray design.
Reciprocal Blast searches were performed to identify the bidirectional best hits between the genomes used in microarray design.
Alternatively, all strains used in microarray design can be used as control if an equimolar mix of their genomic DNA is labeled with the reference fluorochrome and hybridized together with the test.
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dSamples used in microarray analysis.
The sequence that was predicted to detect the most other sequences (as targets) was selected as a probe sequence to be used in the microarray design.
The process was repeated with different random seeds and the number of required probe sequences did not vary significantly while the sequences used in the microarray design could change.
The strains were selected to represent the different biotypes and serotypes of enteropathogenic Yersinia, and also included were three strains (Y. enterocolitica subsp. enterocolitica 8081, Y. enterocolitica subsp. palearctica Y11 [DSM 13030], and Y. pseudotuberculosis IP32953) used in the microarray design.
salmonis ESTs (> 100 bp) were obtained from GenBank and assembled into contigs, providing a further 10,056 annotated (BLASTx e-value <10-4) and 2,526 un-annotated target sequences for the design of oligo probes to be used in the microarray designs (Table 1).
Although comparable data is available from the GNF database [ 15], the advantage of our data is that we used in-house designed microarrays[ 35] that represent a large set of genes (N = 25030), and probes for many genes are more sensitive compared to arrays used in the GNF database.
FSp, AS and FSt participated in microarray design and printing.
This approach is also able to identify chromosomal discontinuities which when coupled to MME analysis can identify new sequences and genes not present in any of the sequences used for microarray design.
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