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To determine whether AA had an impact on cell proliferation, we treated the same type of fibroblasts used in microarray assays with increasing concentrations of the drug and monitored the growth curves (Fig. 2a).
Since pre-amplification was not used in microarray assays, miRNA expression levels in microarray were compared to those in miRNA TaqMan Array A without pre-amplification; 84 miRNAs were detected in both microarray and qPCR-array (Ct ≤ 35).
In recent years there has been increasing interest in the use of nanoparticles (NP) coupled with silver staining for diagnostic applications [ 31- 33] due to the higher sensitivities achievable by this approach compared with the fluorescent dyes that are commonly used in microarray assays.
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Twenty of them were from those used in microarrays assays and the others were newly selected.
TaqMan assays were run for 48 genes on each of the 5 RNA samples that were used in microarray and RNA-seq assays.
dSamples used in microarray analysis.
Typhimurium LT2 Pat and CobB enzymes were used in proteome microarray assays and subsequent experiments, we verified that the homologous enzymes in E. coli K-12 MG1655 also used RcsB as substrate (Fig. S2A, B).
Because the fluorescent dyes used in most microarray assays have slightly different efficiencies for light emission, the detection efficiencies of the phototubes has some wavelength dependence and hence differ for the different dyes, and because the PMTs exhibit nonlinearities at high and low intensities, the log-ratios measured often exhibit some systematic, intensity-dependent variation.
The hrpL mutant WPP96 was used in this microarray assay, and this mutant was complemented by plasmid phrpL in a previous study [12].
For an mRNA sample of 0.2 μg used in each microarray assay, the total amount of rare, intermediate and abundant species are 0.13 μg, 0.07 μg and 4.8 × 10-3 μg respectively.
In hypoxia case, we used 7 microarray assays (GEO: GSE17796) of bone marrow cells.
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