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Multiple testing methods normally used in microarray analyses to correct for false positives include FDR (false discovery rate) and Q-value [51] [53].
In all samples to be used in microarray analyses, RNA quality was assessed by electrophoresis in 2% agarose gels (Invitrogen Life Technologies, Carlsbad, CA, USA), containing 0.5 μg/ml ethidium bromide (Sigma-Aldrich, St . Louis MO, USA).
b Mean of qRT-PCR measurements of the same RNA used in microarray analyses.
Several protocols based on this mechanism have been developed and used in microarray analyses [ 16, 22- 28].
As this method is widely used in microarray analyses we believe that a more in-depth and mathematical description of this method is not warranted for this manuscript.
Lignin content was determined in samples from the four trees used in microarray analyses and the four trees used in qRT-PCR.
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RF-based variable importance measures were previously used in microarray expression data analyses to select a relatively small set of informative genes for disease/sample classification [ 14, 15].
Comparative expression analysis is often used in microarray-based analyses of expression changes due to systematic perturbation to find overlapping effects on biological pathways.
In contrast to eukaryotic mRNA, techniques to amplify bacterial RNA for use in microarray analyses have not been available until recently, and crucially have not been evaluated for M. tuberculosis in well characterised models in which differential gene expression is well described and validated.
Total RNA extracted from the GFP and the two UCH L1 siRNA-expressing cell lines were used in microarray and QRT-PCR analyses.
Importantly, total RNA pools used in microarray and qRT-PCR analyses were isolated in independent experiments.
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