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Have your techniques been used in live experiments?
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During the last few decades, many fluorescent sensors have been developed and used in live cell experiments.
Cultures were infected with the Flx1.8/MitoEYFP lentivirus [9] and used in live imaging experiments.
Cells used in live imaging experiments were incubated at 37 °C under 5% CO2 during imaging.
Animals sorted for GFP and tagRFP expression were used in live imaging experiments beginning at 36 hpf.
Fish used in live imaging experiments were maintained in embryo media containing 0.003% 1-phenyl 2-thiourea (PTU) starting at 8 24 h post fertilization (h.p.f).
While TSQ improved upon earlier histological stains, it was never successfully used in live-cell experiments.
Three to 8 week-old heterozygous CX3CR1+/GFP animals, created by breeding CX3CR1GFP/GFP mice to wild type C57BL/6, mice, were used in live-cell imaging experiments and for immunohistochemical studies.
A more stable conformationally strained TCO (s-TCO) has been used in live cell imaging and radiolabelling of biomacromolecules, but not in pretargeted experiments in live animals [47 50].
We would like to thank Lara Pickle for invaluable assistance with tissue culture and Donald Hutson for his technical expertise in the construction and ongoing maintenance of the microscope stage-top incubator used in our live imaging experiments.
The B. impatiens used in this experiment had experience foraging on live M. guttatus, but no live plants were available to them during the trial periods.
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