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The primers used for vector construction are shown in Additional file 1: Table S1.
E. coli strain DH5α (Toyobo, Osaka, Japan) was used for vector construction and cloning.
Escherichia coli strain DH5α was used for vector construction and grown in LB broth at 37 °C.
Primers used for vector construction are available in Table S1.
The BamHI, SpeI or KpnI restriction sites used for vector construction are underlined.
The TaCBLs and TaCIPKs primers used for vector construction are listed in Additional file 2: Table S1.
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The PCR primers used for vectors construction and genomic PCR selection are listed in Supplementary Table S1.
Bacterial strains and plasmids used in this work are listed in Table 1, and the primers are listed in Table 2. E. coli JM109 was routinely used for vectors construction, E. coli TOP10 bearing pAN2 for methylation of pMTL007 deprived plasmids, and E. coli ER2275 bearing pAN1 for methylation of pIMP1.
The E. coli JM109 strain (Novagen, USA) was used for vectors construction.
Sequences of primers used for ALSV vector construction.
Primers used for sponge vector construction are described in Supplementary Table 1.
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