Sentence examples for used for transient expression from inspiring English sources

As a control, a construct constitutively expressing GFP alone was used for transient expression in tobacco protoplasts.

Two plasmids were used for transient expression.

The constructs were electroporated into Agrobacterium tumefaciens GV3101 and the bacteria used for transient expression assays.

This method can be used for transient expression of transfected genes for induction of regeneration (3).

Chinese Hamster ovary (CHO-K1) cells cultured in Ham's F12 medium (Invitrogen, Merelbeke, Belgium), were used for transient expression experiments.

The 35S promoter was subsequently replaced by the putative AtIPS1 promoter (1000 bp upstream the initiation codon), and the resulting construct was used for transient expression assays.

Both expression constructs were used for transient plant expression of PfGAP50.

HEK293T, a human embryonic kidney cell line transfected with the SV40 large T-Ag (SV40TtsA1609) [22], was used for transient eukaryotic expression.

Potato cultivars Katahdin, Atlantic, Megachip, USW1 (a haploid clone derived from Katahdin), and a diploid wild potato species S. bulbocastanum were used for transient gene expression experiments.

The constructs used for transient gene expression and localization studies were derived from binary vector pK7FWG2, which allows C-terminal fusion of protein of choice with GFP [43].

In our experiments, a disarmed strain of A. tumefaciens that lacked the ability to transform plant cells with auxin biosynthesis genes suppressed P. syringae-induced SA synthesis when inoculated at densities typically used for transient gene expression, and similar results were obtained with strains that lacked the Ti plasmid or carried a mutation in virG.