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was not used for transcripts that had signals extending largely to the contralateral side.
Biases due to variations in genotype, developmental stage and type of the tissue used for transcripts isolation, and sequencing technologies may account for the observed differences.
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Briefly, 1 μg of total RNA was reverse-transcribed using random primers and reverse transcriptase (Invitrogen) and exposing the samples to 65°C for 5 min, followed by 42°C for 60 min and 75°C for 15 min. Of the resulting cDNA, 1 μl was used for transcript-specific PCR with Taq DNA polymerase (Taq Core Kit, Q-Biogene).
Scripture was used for transcript reconstruction.
ATH1 array was used for transcript profiling.
RNA samples were used for transcript sequencing and SNP calling.
The same technique was used for transcript analyses.
These reads were then used for transcript assembly.
Primers used for transcript map development [ 6] were then used to identify BAC clones.
Microarrays developed for a specific species have been used for transcript profiling of closely related species.
Only contigs containing more than two ESTs were used for transcript profiling.
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