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Scripture was used for transcript reconstruction.
ATH1 array was used for transcript profiling.
RNA samples were used for transcript sequencing and SNP calling.
The same technique was used for transcript analyses.
These reads were then used for transcript assembly.
As in [ 104], only contigs containing more than five ESTs were used for transcript profiling.
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Briefly, 1 μg of total RNA was reverse-transcribed using random primers and reverse transcriptase (Invitrogen) and exposing the samples to 65°C for 5 min, followed by 42°C for 60 min and 75°C for 15 min. Of the resulting cDNA, 1 μl was used for transcript-specific PCR with Taq DNA polymerase (Taq Core Kit, Q-Biogene).
was not used for transcripts that had signals extending largely to the contralateral side.
Biases due to variations in genotype, developmental stage and type of the tissue used for transcripts isolation, and sequencing technologies may account for the observed differences.
The time points used for global transcript analysis are shown in the bacterial growth curves presented in Fig. 1.
The primer pairs used for viral transcript quantification and internal standard GADPH are listed in Table S2.
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