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Different thresholds are used for this method.
Two different importance sampling distributions can be used for this method.
Techniques used for this method are solely based on statistics, e.g., Weibull analysis and log-normal and Poisson laws [34].
Different to the RT-qPCR technique, the RNA samples used for this method were ligated with 5′-adaptors and 3′-adaptors before the reverse transcription reaction.
Since the distribution of the RBM over the skeleton is both age- and patient-specific, ideally, this fraction should be individually measured, and a patient-specific percentage of the RBM in a certain VOI should be used for this method.
We noticed that some Ni-NTA lots contained less than 1% spherical beads, in contrast to ∼93% in 'good' lots, and these beads could not be used for this method.
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You will need a metal coat hanger to use for this method.
The number of rope pieces you use for this method can vary as long as you have four strands to work with.
For explanation of the 12 genes used for this analysis, see Methods: Statistical Analyses.
Table S2 shows the primers sets used for this study (supplementary methods).
Detailed information of the methods used for this study is described in Supplementary methods.
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