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Alternatively, a reference point for the classification of toxicity may be calculated by procedures similar to those used for the benchmark dose approach in vivo.
The Input and Output data image is as shown in Additional file 1: Appendix 1 while the program script used for the benchmark test is given in Additional file 2: Appendix 2. We created a Hadoop cluster on Amazon EC2, composed of one master for the master name and job tracker node, and varying numbers of slave nodes for task tracker and data nodes.
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13321_2013_478_MOESM1_ESM.docx Additional file 1: Table S1: Physicochemical properties of amino acids residues, setup of all software tools used for the benchmarking study.
Detailing the diseases and variants used for the benchmarking of the 50 exomes in Fig. 1.
This merged reference set was written to a file and subsequently used for the benchmarking.
The diseases and variants used for the benchmarking of the 50 exomes in Fig. 1 are detailed in Additional file 1.
The variables used for the benchmarking and quality indicator calculations were chosen because of their capacity to describe clinically important and/or modifiable processes and outcome [ 11].
As a synthetic scenario in which the real history of insertion and deletion events is known, we worked with one of the simulated dataset previously used for the benchmarking of trimming methods (Capella-Gutierrez et al., 2009).
The ChIP-seq of histone modification data from Mikkelsen et al [ 18] (the raw data can be downloaded at ftp://ftp.broad.mit.edu/pub/papers/chipseq/Mikkelsen2007/alignments/) including H3K27me3 (K27), H3K4me3 (K4) and H3K36me3 (K36) were used for the benchmarking test to evaluate the performance of our program QChIPat.
It might be argued that it is not possible to achieve a symmetrical distribution with general questions about patient satisfaction [ 32, 33], rendering the selection of the questions used for the national benchmark disputable.
The scripts and commands used for this benchmark are available in the Supplementary material.
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