Sentence examples for used for the array from inspiring English sources
These experiments included an additional cell line from a Golden Retriever hemangiosarcoma (Emma) that was recently developed and therefore not used for the array experiments, but allowed us to validate gene set enrichment in an independent sample.
Twenty of them, 12 male and 8 female, were used for the array screening assays and the rest for the real time quantitative PCR validation.
From the same original total RNAs used for the array experiments, cDNA was generated from 1 µg RNA using SuperScript II reverse transcriptase (Invitrogen).
Array-CGH has the potential to analyze alterations of DNA copy number on a genome-wide scale with relatively high resolution, depending on whether BACs, PCR products or oligonucleotides are used for the array elements.
The more robust and sustained decrease in N-myc levels in these cells versus those used for the array may explain the somewhat more pronounced reductions in expression of klf2, klf4, lif, and lin28b.
Matrix metalloproteinase 7 (MMP7) [14] showed the highest fold-change in Wnt-associated genes and was confirmed by RT-PCR on the cDNA used for the array analysis (DN 40.09±23.88, LD: 1.0±1.73 (p<0.05)) as well as on an independent cohort of patients with DN (DN: 6.45±6.62; LD: 1.00±0.79 (p<0.05)) (Fig. 5a).
Validation of BIC expression was conducted by real time PCR (RT-PCR) using miRNA/RNA preparations from donors which were independent of the original 10 healthy human volunteers used for the array-based expression profiling.
We, thus, assumed that expression changes with no statistical significance may have biological significance and could become statistically significant if, for example, six chips instead of three were used for the arrays.
About 2 µg of total RNAs isolated from the small intestinal smooth muscles of the smDicer mutant and the WT control mice (2 each) were used for the arrays.
The annealed paired oligos are then ligated and PCR used for the array-based detection.
Nick translation labelled, large genomic insert clones from the library used for the array-CGH [ 17] with about 1 Mb spacing were used to map the translocation breakpoints of the involved chromosomes.
