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Both HeLa cells and hCSCs were used for successive experiments.
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When the number of specimens was insufficient we used smaller replicas, and when there was no mortality the same control could be re-used for successive experiments, occasionally with less than ten individuals (Additional file 1: Table S2).
All the media used for the successive experiments were supplemented with constant concentrations of these inducers.
The microwave boiling in T-EG buffer for 15 min was found to be the optimal antigen retrieval method for both anti-GAGE-1-8, MAGE-A1 aNY-ESO-1mAbsand and was used in the successive experiments.
Male mice were used for all experiments.
These cells were used for subsequent experiments.
Female adults were used for all experiments.
Zhonghua 11 was used for all experiments.
P1-P3 cells were used for all experiments.
This buffer was used for all experiments.
Nipponbare was used for all experiments.
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