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Generalized gradient approximation is used for structural study, whereas local spin density approximation is used for electronic calculation.
The fact that our 39AA fusion construct can be re-activated by Mg-ADP (Fig. S1B) suggests this construct can be used for structural study on the Mg-ADP activation mechanism.
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For example, DDAO was used for structural studies on rhodopsin, CHAPSO is able to maintain the native structures of CCR5 and CXCR4, and LDAO has been used for solubilizing human leukotriene B4 receptor [62], [63], [64], [65].
Interestingly both parameters were dependent on the nature of the mutant and a stable deglycosylated mutant N201D of bCD38 could be produced that can be further used for structural studies.
The K48-linked diUb used for structural studies was produced as described elsewhere [36].
Heterologous co-expression of LdTopIL and LdTopIS genes in a topoisomerase I deficient yeast strain, reconstitutes a fully functional enzyme LdTopIL/S which can be used for structural studies.
The His-tags were removed from the proteins used for structural studies by thrombin cleavage.
H25 was used for structural studies and is identical to H27 but lacks the two terminal 3′ bases.
To identify optimal conditions for solution nuclear magnetic resonance (NMR) studies of Cav3, we assessed the H N TROSY NMR spectrum of Cav3 in the presence of 10 different detergents commonly used for structural studies of integral membrane proteins.
Sequences of Mtb rrnA AP3 ( Gonzalez-y-Merchand et al., 1996 ) and Tth 23S ribosomal RNA (rRNA) ( Hartmann et al., 1987 ), promoters used in in vitro assays, and full con ( Gaal et al., 2001 ) used for structural studies.
The results led to the maturation of the technique and since then IM-MS has been used for structural studies of several different biomolecules, such as proteins [ 18– 20], lipids [ 21– 26], carbohydrates [ 27– 30] and even intact viruses [ 31– 31].
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