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Chinese Hamster Ovary (CHO) cells are also widely used for stable expression of proteins, but are inefficient in protein expression by transient transfection [6].
Lentiviral infection was used for stable expression.
To date, various strong enhancers/promoters have been used for stable expression of transgenes in animal cells.
The pDSRed-2 vector (Clontech Laboratories, Inc., Palo Alto, CA) was used for stable expression of red fluorescent protein (RFP) in the human pancreatic cancer cell line XPA-1.
In brief, NHE1-null LAP1 cells developed from parental Ltk-mouse muscle fibroblasts [ 16] were used for stable expression of wild-type NHE1 (LAPN cells) or expression of NHE1-E266I containing a single point substitution of glutamate266 for isoleucine that results in complete loss of ion translocation activity (LAPE) [ 5, 6].
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SPRY2 expression construct used for stable and transient expression was a kind gift from Graeme Guy (Institute of Molecular and Cell Biology, Singapore).
This plasmid was used for stable as well as transient expression of NOF1 GFP.
At the time agroinfiltration was developed, the building blocks of plant-based expression cassettes used for stable transformation were regularly drawn from plant pathogens in recognition of their ability to subvert host controls over gene expression (Fig. 1a).
Conversely, lentiviral transfer of short hairpin RNA (shRNA) could be used for stable and specific reduction of CD38 expression in high-risk CLL.
The same recombinant A. tumefaciens strains, used for transient expression in N. benthamiana, were used for stable transformation (either pBIN-CYP98A22 or CYP98A3).
A retroviral cDNA expression library generated from RAW264.7 macrophages cloned into the retroviral vector pFB-Neo (Stratagene) was used for stable transduction of NIH3T3 cells as described [24].
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