Sentence examples for used for sgRNA expression from inspiring English sources

The tRNATyr promoter was used for sgRNA expression.

The pDR274 vector (Addgene Plasmid 42250), harboring a T7 promoter positioned upstream of a partial guide RNA sequence (Hwang et al., 2013b) was used for sgRNA expression.

These cassettes can be directly electroporated as unpurified PCR products into Ciona embryos for sgRNA expression in vivo, resulting in high frequency of CRISPR/Cas9-mediated mutagenesis in somatic cells of electroporated embryos.

Several reports have suggested that the first nucleotide of the target sequence should be 'G', if the U6 promoter was used to drive sgRNA expression (Li et al. [2013b]; Mali et al. [2013]).

The U6 promoter has been used to drive sgRNA expression (Chiu et al. 2013; Dickinson et al. 2013; Friedland et al. 2013; Katic and Grosshans 2013; Waaijers et al. 2013).

Notably, tRNATyr was inefficient at targeting in ATCC4124 though it was effective in S288C diploid (Supplementary file 1A), indicating that the choice of tRNA used for the expression of the HDV-sgRNA impacts multiplexing in a strain-dependent manner.

Two plasmids were used for transient expression.

The primer sequences used for the construction of sgRNA expression plasmids are listed in Table S1.

The oligonucleotides used for construction of each sgRNA are listed in Table 1.

(A ) The construct for CLTA1 sgRNA expression (top) was designed to generate transcripts containing the original Cas9-binding sequence v1.0 (Jinek et al., 2012 ), or sequences extended by 4 base pairs (v2.1) or 10 base pairs (v2.2). (B ) Surveyor nuclease assay of genomic DNA isolated from HEK293T cells expressing Cas9 and/or CLTA sgRNA v1.0, v2.1 or v2.2.

Four pairs of isocaudamers were used for assembling the U6P-sgRNA cassette in the pEntry vectors (Additional file 1: Figure S2, Additional file 1: Figure S3).