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EMBOSS version 6.3.1 was used for sequence manipulation [ 108].
CONSED [ 134] was used for sequence manipulation that included read/contig editing, primer design, and finish read processing.
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Programs of the Sequence manipulation suite [ 70] version 2 [ 71] have been used for general sequence manipulation, assembly and translation tasks.
PCR primers were used for sequencing.
The extracts were further used for sequencing.
We used the alignment editors BioEdit version 7.0.8 (T. Hall, Ibis Biosciences) and Jalview version 2.2.1 (60) for sequence manipulation.
Utilities from the Sequence Manipulation Suite [ 37] were used for routine sequence handling.
Other packages that can be used include: SeqLogo for visualization of PWM, GenomeGraphs [56] for further graphics functionality, BiomaRt for retrieving annotations, IRanges and GenomicRanges for interval manipulations, Biostrings for sequence manipulations, etc.
The EMBOSS package [ 58] was used for routine manipulation of sequences.
The DNASTAR program was used for the preliminary sequence manipulations.
The alignments were formatted using the Sequence Manipulation Suite (Stothard, 2000 ) web server.
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