Sentence examples for used for sequence determination from inspiring English sources

pBac17 DNA as well as cesium chloride preparation of pVT1 DNA from V. tapetis were used for sequence determination.

MPSS is akin to SAGE in that it uses short sequence tags to identify transcripts, however, the techniques differ in the method used for sequence determination.

The Enthelechon backtranslation tool (Markus Fischer, Backtranslation Tool, http://www.entelechon.com/backtranslation, Entelechon GmbH, Regensburg, Germany) was used for codon sequence determination.

MS/MS spectra and summaries of all digested fragments used for the sequence determination of all cyclotides from V. ignobilis are available as Supplementary Data (Supplementary Fig. S1, Supplementary Fig. S2, Supplementary Fig. S3, Supplementary Fig. S4, Supplementary Table S1, Supplementary Table S2).

Both pBac17 and pVT1 were used as DNA sources for sequence determination.

PCR primers were used for sequencing.

The extracts were further used for sequencing.

For sequence determination we used a shotgun base approach called LoPPS (Long PCR Product Sequencing) [16], [17].

The resulting PCR products were directly ligated into a pGEM vector using a pGEM-T-easy cloning kit (Promega) for sequence determination.

Thus use of bar coding allowed many samples to be pooled for sequence determination, then the reads could be sorted into individual experiments by bar code.

To ensure the sequence authenticity, three clones were randomly selected for sequence determination.