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GO, KEGG and EC based tools and scripts were used for sequence annotation.
CLC Main Workbench was used for sequence annotation and in silico cloning (CLC-bi, Denmark).
The semi-automated pipeline TriAnnot was used for sequence annotation [ 23] and the annotation was curated under Artemis v.11 [ 24].
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The software package DNAMAN 7 (Lynnon Biosoft, USA) was used for sequence annotations, including nucleotide statistics and ORF searching.
The program can be used for sequence editing, annotation of sequence features, handling of output from high throughput sequencers, or from BLAST searches, as well as for various population genetic analyses.
The Staden sequence analysis package [ 18] was used for sequence assembly and annotation of C. harmani mitogenome.
The GigasDatabase http://public-contigbrowser.sigenae.org:9090/Crassostrea_gigas/index.html has been used for sequence processing, contig assembly, annotation, and project data hosting.
Genes were predicted with Prokka [ 52], Artemis was used for sequence visualisation and manual editing of annotations [ 53], and RATT was used to transfer annotations between genomes with similar cassettes [ 54].
The reads selected each generated a single scaffold for the mt genomes and the reference genome CBS 6340T was used for all sequence annotation.
All annotation data were integrated with the Generic Genome Browser (GBrowse), a genome viewer that is widely used for visualization of sequence annotation (27).
Although they use standardized formats, such as fasta and gff3, for data presentation and distribution, each center uses its own data formats for sequences, annotation data, and other chromosomal information.
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