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It is used for sequence analyses, protein expression, and microarray analyses.
We found that the sequences from NCBI included all sequences from UniProtKB/Swiss-Prot and DDBJ; therefore, only the NCBI data were used for sequence analyses.
All 85 proteins were used for sequence analyses.
Both Geneious bioinformatics software (Biomatters) and Sequencher 4.5 (Gene Codes) were used for sequence analyses.
Primers used for sequence analyses are listed in Supplementary Table S4.
Description of the loci, specific primers for their amplification, PCR conditions, procedures for DNA sequencing and software used for sequence analyses can be found in [ 6].
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The primers used for sequencing analyses were IFNγ Forward (5'-TAT GAT TCT GGC TAA GGA-3'), IFNγ Reverse (5'-CCC CAA TGG TAC AGG TTT CT-3') and IL-10 Forward (5'-TGT GGA AGG GGA AGG TG-3'), IL-10 Reverse (5'-TAA AAG ATG GGG TGG AAG AAG3').
Orthologs of imprinted genes and their paralogs were identified according to the gene-tree topology and used for sequence rate analyses and expression assays.
Accession numbers of RECKs used for sequence alignment and phylogenetic analyses are as follows: Tribolium (XP_974450), Nematostella vectensis (XP_001635685), Drosophila melanogaster (NP_648733), Apis mellifera (XP_392031); Aedes aegypti (XP_001657759); Homo sapiens (NP_066934).
MEGA4 was used for sequence alignments and phylogenetic analyses (both neighbor-joining and maximum parsimony approaches were applied) [ 23].
The ClustalX software [ 37] was used for sequence alignments which were further analysed in the program Genedoc http://www.nrbsc.org/gfx/genedoc/index.html.html
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