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CLUSTALW was used for sequence alignments [15]; phylogenetic trees were built using Phylip.
After initial BLAST analysis, all available sequences for the specific targets were used for sequence alignments.
GenBank accession numbers of Usutu virus polyproteins used for sequence alignments are shown in Table 1.
SeqManPro (Lasergene 8.0, DNASTAR) was also used for sequence alignments containing BAC assembly consensus sequences and predicted gene sequences.
MEGA4 was used for sequence alignments and phylogenetic analyses (both neighbor-joining and maximum parsimony approaches were applied) [ 23].
For the Japanese eel, the sequences of scaffolds anchored to chromosomes (linkage groups) were used for sequence alignments.
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Of those, ~80% of the reads were used for sequence alignment, while 20% of sequence reads were not aligned.
Geneious version 6.1.6 (Biomatters Limited) was used for sequence alignment and visualization.
Covers principles and methods used for sequence alignment, motif finding, structural modeling, structure prediction and network modeling.
Presents principles and methods used for sequence alignment, motif finding, expression array analysis, structural modeling, structure design and prediction, and network analysis and modeling.
For the molecular analyses, partial sequences of the COI, 16 S rRNA, and 28 S rRNA gene fragments were obtained and afterwards checked using a sequence alignment editor (BioEdit v. 7.2.5 37 as described in Bolotov et al.8 The PCR primers are provided in Supplementary Table 4. Muscle algorithm implemented in MEGA638 was used for sequence alignment of COI, 16 S rRNA and 28 S rRNA gene fragments.
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