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Exact(8)
The mixtures were kept overnight, and the resulting extracts were filtered to remove any solid residue and were used for sensitization.
Separately, CZTS nanoparticles were synthesized from high temperature arrested precipitation and subsequently used for sensitization of ZnO/ZnS CS-VANR nanostructures.
In this review, examples of animal models are described, in which recombinant allergens have been used for sensitization and/or treatment of allergic responses and how they have been used to enhance our understanding of the pathology of allergic diseases.
It has been shown previously that lipopolysaccharide (LPS) is a constituent of CAPs as well as OVA, which was used for sensitization (Campbell et al. 2005).
Although different allergen mixtures are used for sensitization and challenge, we have previously shown that HDE immunization specifically induces an allergic response to CRA[ 12].
Equally important is the observation that the antigen used for tolerization must be the same as that used for sensitization and challenge, in order to have a detectable effect in vivo.
Similar(52)
Thus, the gene expression data presented here in our study corresponds only to the effects of the food allergens used for sensitizations that may mimic the actual allergic responses in humans.
Briefly, animals (ten female BALB/c mice aged 6 - 8 weeks purchased from Harlan Winkelmann, Borchen, Germany) were sensitized by subcutaneous injection of 10 μg OVA (grade VI, Sigma, Steinheim, Germany, containing 1 ng of endotoxin per 10 μg OVA [dose used for sensitization]) in 200 μL phosphate buffered saline (PBS, Sigma, Steinheim, Germany) or sham injections of PBS on days 0, 7 and 14.
The optimized molar ratio of N3/N719 was used for the sensitization of hybrid photonodes.
The TCA-soluble fraction was used for the sensitization of microplates to IgM (ELISA-IgM) antibodies [ 26, 28].
This total extract was used for the sensitization of microplates to IgG (ELISA-IgG) antibodies [ 27, 28].
Related(20)
used for education
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