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Pickering et al. have described a simple method that can be used for samples of irregular thickness, such as plant tissues.
Total sample RNA was extracted using commercial kits as appropriate to the sample type; QIAamp Viral RNA Mini spin protocol (Qiagen, Crawley, UK) was used for samples of low cellularity (swabs and BALs), RNeasy Mini kit (Qiagen, Crawley, UK) was used for fresh or frozen tissue samples and RecoverAll™ Total Nucleic Acid Isolation Kit (Applied Biosystems, Warrington, UK) was used for FFPE sections.
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Gamma-irradiated, clean and sterilized bottles (200 ml capacity) were used for sampling of water from different zones of Delhi, India.
One of the tanks was later lead-plated, sealed, equipped with self-contained air supply, and used for sampling of fission products in the post-blast debris after the Trinity test.
Another method used for sampling of interstitial fluid from human tumors is tissue elution [10].
The Navrongo HDSS database was used for sampling of prospective participants in this study.
VABB can be performed under stereotactic or ultrasonographic guidance; an 11-Gauge (11G) needle is most commonly used for sampling of the suspicious lesion [ 1].
These PM1-like sequences were found in locations far removed from where strain PM1 was inoculated and in samples collected with sterilized equipment that had not been used for sampling of inoculated plots.
Ten runs of 20 cycles were used for samples; 5 runs of 10 cycles for blanks.
Equal amounts of RNA were used for samples before and after treatment of the same subject.
Methods used for sampling, preparation of hair samples, and chemical analysis are described extensively elsewhere (Huel et al. 1984).
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