Three tubes of LJ medium (Fluka Analytical, Sigma-Aldrich, Switzerland) (two tubes with glycerol and one with sodium pyruvate) were used for inoculation and samples were incubated at 35 ± 2°C.
Concentrated samples used for mouse inoculation were assayed for PrPCWD by serial PMCA (sPMCA) over three rounds of amplification.
This late seroconversion in bank voles may have been caused by relatively low doses of virus in bank vole saliva, urine, and feces samples used for intranasal inoculation.
The nasal-wash samples were used for intranasal inoculation of the ferrets for the subsequent passages 7 through 10.
Sabouraud dextrose agar and Dermasel media were used for the inoculation of the samples.
TK SLC-L liquid medium, which is used for the inoculation of clinical samples, contains selective antimicrobials to minimise contamination.
B. cereus R2SK spores, which were prepared on the FNA sporulation medium [ 41] for 7 days at 25°C, were used for the inoculation of natural samples.
B. cereus R2SK vegetative cells used for the inoculation of natural samples were grown in Gordon's medium containing soil extracts [ 42]; however, only the first soil infusion step was performed.
The SM2 304 wpi plasma sample used for inoculation of SM7, SM8, and SM9 contained a mixture of SIVsmm nef variants that did (cluster A, n = 17/31) or did not (cluster B, n = 14/31) downmodulate TCR-CD3.
Samples were previously used for intrapleural inoculation in rats (12 asbestos and 5 synthetic mineral fibers) [ 74, 76, 86].
