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Preconditioned C18 Bond Elut columns (Varian, Inc., Palo Alto, California, US) are used for sample extraction.
A CREST 1875HTAG ultrasonic processor (CREST, USA) was used for sample extraction.
The dry blank Twister® was not used for sample extraction but was stored together with the rest of the batch, ensuring the detection of any contamination that could have occurred during conditioning, storage or anything that was not related to sample preparation.
A multi-stage method was used for sample extraction.
The different solvents (HPLC grade) and buffer salt used for sample extraction or chromatographic methods were purchased from Baker Ind. (Phillipsburg, USA).
HPLC-grade solvents (acetonitrile and methanol) were from Biosolve (Valkenswaard, The Netherlands), and glacial acetic acid and all other chemicals used for sample extraction and preparation were from Sigma-Aldrich (St . Louis MO, USA).
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Separate laboratories were used for sample preparation, DNA extraction and PCR amplification.
Different rooms were used for sample preparation, DNA extraction, and setting up PCR.
Solvents used for sample preparation and extractions were of analytical grade, while those for HPLC analysis (methyl-t-butyl ether, methanol, ethyl acetate and tetrahydrofuran) were of HPLC grade; all were obtained from Merck (Darmstadt, Germany).
Despite these advantages, several Taq Polymerase inhibitors are found in these clinical specimens or they are commonly used for sample collection and DNA extraction, like EDTA, Proteinase K, Phenol and high salts concentrations [ 61, 62].
Extraction methods used for sample preparation include gas or liquid chromatography (GC or LC) and capillary electrophoresis (CE).
More suggestions(15)
used for sample heating
used for sample processing
used for uranium extraction
used for road extraction
used for juice extraction
used for sample mineralization
used for pigment extraction
used for factor extraction
used for sample treatment
used for sample classification
used for sample quantification
used for sample digestion
used for sample derivatization
used for sample detection
used for sample identification
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