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Analytically graded 65% HNO3 and 30%H2O22 (Merck, Germany) were used for sample digestion.
A closed-vessel MWS-3+ microwave system (Berghof, Germany) with temperature control mode was used for sample digestion.
The further purified guaranteed-reagent (GR) HNO3 using a sub-boiling DuoPUR acid purification system (Milestone) and the metal-oxide-semiconductors reagent HF were used for sample digestion (Li et al. 2014).
AAS-based techniques can be used if high temperatures are used for sample digestion (for instance dry ashing at 450°C), as shown by L21.
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One reason is probably the complexity of the workflow used for sample preparation, involving trypsin digestion and, often, an immunoaffinity step for target peptide enrichment [ 8– 12].
A microwave-assisted digestion method was used for sample preparation.
Rational selection of the acid combinations used for various sample digestions is very important to achieve the reliable analytical method.
However, the 51 sheep and 3 goat isolates analyzed gave positive results with the highly sensitive WB method (TeSeE Bio-Rad), which used the classic pK concentration for sample digestion and Sha31 MAb for PrPres detection.
Experimental conditions for sample digestion using nitric acid, hydrogen peroxide and a reflux system by "cold finger" were optimized employing a two-level full factorial design and a Box-Behnken design.
This paper presents studies involving the determination of Hg in biological samples by cold vapor atomic absorption spectrometry (CV-AAS) using a simple and inexpensive system for sample digestion.
Experimental designs for seven factors (HNO3, H2SO4 and H2O2 volumes, digestion time, pre-digestion time, temperature of the hot plate and sample weight) were used for optimization of sample digestion procedures.
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