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Quantitative real-time reverse-transcriptase (RT) PCR was also used for quantitation of virus in PBS, plasma or buffy coats (a total of 140 μl).
The standard calibration technique (SCT) was used for quantitation.
The same three peptides were used for quantitation of each protein across all replicates after abundances were normalized to ppm.
DPV was used for quantitation of hydrazine in water samples by the standard addition method.
Flame atomic absorption spectrometry using an acetylene-air flame was used for quantitation of the analyte after preconcentration.
These data suggest that the assay is standardized appropriately against the WHO standard across its linear range and can be used for quantitation of HCV.
For QC-RT-PCR, a competitor RNA molecule having a deletion was designed and used for quantitation of the BRSV viral mRNA.
Response factors generated from authentic monosaccharide standards were used for quantitation of pneumococcal polysaccharides and conjugates with confirmation of peak assignments by retention time and mass spectral analysis.
An analytical platform comprising reverse-phase chromatographic separation followed by mass spectrometric detection by positive electrospray ionization in multiple reaction monitoring was used for quantitation of UPA within 7 min.
The data were recorded, processed and used for quantitation of individual compounds with Xcalibur® software.
The ALT and AST kit were used for quantitation of transaminase in mice serum of different groups.
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