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The ∆∆Ct method was used for quantification.
Confocal stacks spanning the entire islet were used for quantification.
Yellow boxes denote the ROI used for quantification of muscle contractions.
Only spectra in which all the expected iTRAQ reporter ions were detected were used for quantification.
A standard curve generated from a pool of all cDNA samples was used for quantification.
(C) Representative CD31 immunofluorescence stained section used for quantification (scale bars: 20 μm).
Yellow box indicates ROI used for quantification of displacement due to muscle contraction.
Only images that captured the entire islet were used for quantification.
ImageJ (National Institutes of Health) was used for quantification of the immunolabeled nuclei.
Software used for quantification and data analysis is indicated under experimental details in Methods.
Flow Logic software was used for quantification of the percentage of S-phase cells.
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CEO of Professional Science Editing for Scientists @ prosciediting.com