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These recombinant proteins were used for pull down experiments from mouse brain lysates.
Firstly, the same amount of empty beads were used for pull down to ensure only specific binding partners were analyzed.
For ubiquitylated protein capture, 200 μg of mitochondrial protein extracts was used for pull down with HALO-UBAUBQLN1 resin as described previously (Kazlauskaite et al, 2015).
To identify interaction partners of Sec7, GST-Sec7 N-terminus, GST-Sec7 domain, GST-Sec7 C-terminus and GST for control were bound to glutathione sepharose 4B beads (GE Healthcare) and used for pull down assays.
High 5 (Life Technologies) insect cells were co infected with baculoviruses encoding the respective PI3K subunits, lysed after 72 hr, and complexes were recovered on glutathione agarose, evaluated by gel electrophoresis and either used for pull down experiments on beads, or cleaved off overnight with HRV 3C protease, gel-filtrated and snap frozen.
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It's basically a Y-shaped piece of webbing that can be attached to a bar or branch and used for pull-ups, supporting feet for press-ups or other exercises.
Invented by an American Marine, it's a way of keeping fit without going to the gym and is basically a Y-shaped piece of webbing which can be attached to a bar or branch and used for pull-ups, supporting feet for press-ups or other exercises.
They were used for pulling heavy artillery, transporting supplies and the wounded – and for riding.
Ponies are used for pulling carriages and pack loads and as children's riding horses or pets.
That horses were long used for pulling chariots rather than for riding is probably attributable to the horse's inadequate strength and incomplete domestication.
These include bleeding cups (used in bloodletting), a wrench used for pulling teeth and, of course, an amputation saw -- all of which will likely make you think twice before complaining about next year's flu shot.
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CEO of Professional Science Editing for Scientists @ prosciediting.com