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Our other approach to assess MM tolerance was to hybridize the array to genomic DNA from organisms with varying degrees of relatedness to the four target strains used for probe design.
We found that a different E. coli strain from the two used for probe design still gave good results, with 100% of gene families detected by one or more probes.
saurensis that were used for probe design.
All these ESTs were also used for probe design.
Over 36,000 EST sequences from eleven rhesus tissues were used for probe design.
NM wrote the software used for probe design, designed microarray probes and performed microarray hybridizations.
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Additional file 4: Supplementary data S4: list of genome sequences and respective accession numbers used for probes design.
DNA sequences specific exclusively to L. brevis DNA and RNA were selected and used for probe and target DNA design.
Finally, a selected set of 15,253 predicted transcript sequences was used as targets for probe design of a comprehensive P. pastoris microarray.
Terminal exons were used whenever possible for probe design due to their proximity to the 3' end of a gene and because they do not cross intron/exon DNA sequence boundaries.
Primers were designed from the barley sequences used for microarray probe design (Supplementary Data 2, available online).
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used for waveform design
used for training design
used for control design
used for planning design
used for prediction design
used for logic design
used for participatory design
used for probe labelling
used for probe annotation
used for probe summarisation
used for biosensor design
used for probe transcription
used for quantizer design
used for probe insertion
used for probe summarization
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