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One virulent strain (termed LB2) of PRRSV (collected in our laboratory) was used for positive control.
Concanavalin A (Sigma) was used for positive control wells (5µg/ml final) and media supplemented with 80% DMSO for negative control wells.
A primer pair for the T cell receptor gene (5'-CAAATGTTGCTTGTCTGTG-3' as a forward primer and 5'-GTCAGTCGAGTGCACAGTTT-3' as a reverse primer) was used for positive control of the genomic DNA.
We thank Santjie du Toit for assistance with field observations, Truuske Gerdes of the ARC-OVI for providing the EMC virus used for positive control purposes, Daniel Haydon for review of the draft manuscript, Cromwell Purchase for assisting with dissections, and Chris Oosthuizen and Mashudu Phalanndwa for field trapping.
Heart was used for positive control of PDE3A.
cccp (0.05 mM) was used for positive control.
Similar(37)
A number of wells were used for positive controls, inoculum viability and solvent effect.
Total RNA extracted from human brain was used for positive controls; reactions lacking RT were also included.
The masseter muscles and trigeminal ganglia (used for positive controls of immunolabelling) were quickly removed, post-fixed for 90 min, and immersed in a 20% sucrose solution overnight at 4°C.
Tail DNA was used for positive controls and water for negative control.
Expression at other sites (embryonic brain or skin) was used for positive controls.
More suggestions(15)
used for moisture control
used for positive linear
used for positive effect
used for positive training
used for positive colony
used for positive cytology
used for positive signal
used for positive moment
used for positive weight
used for positive pressure
used for positive selection
used for positive identification
used for positive change
used for biological control
used for negative control
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