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As HGP sequencing efforts accelerated, concerns arose that only four genomes, a couple of which belonged to known laboratory personnel, were being used for physical mapping and sequencing (Marshall 1996b).
The LN54 radiation hybrid panel was used for physical mapping [44].
Morex (which is susceptible to leaf stripe) and Thibaut (the donor of the Rdg2a allele) were used for physical mapping of the locus, leading to the identification of three Rdg2a candidates representing sequence-related members of a gene family.
Several gel-based restriction fingerprinting methods have been used for physical mapping of some genomes from BACs, such as Caenorhabditis elegans and Saccharomyces cerevisiae [6], human [7], Arabidopsis thaliana [8] and rice [9].
The genomic locations of the P. falciparum orthologues were, in turn, used for physical mapping to chromosomal locations in the genome of P. c. chabaudi through the conserved genetic synteny between the human malaria parasite P. falciparum and rodent malaria parasites [33].
Such large discrepancy between linkage map and physical map distances could also come from genome variations, as the resource family used for genetic linkage map is not related with the fish used for physical mapping.
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Poursarebani et al. [ 38] used for physical map building BAC clones with 6 68 sequence tags.
Shotgun clones are also used for physical map construction but are less robust than BAC-by-BAC characterization.
Fingerprints of 74,013 (87%) clones were validated and used for physical map assembly, corresponding to approximately 11.9-fold coverage of the turkey genome.
The present study provides a set of validated gene-based markers for common bean that are derived from G19833, an Andean landrace that is an important source of disease and abiotic stress tolerance which has been used for physical map development and as a mapping parent.
Besides contaminations and clones with too few or too many fingerprint fragments, empty vector clones or non-viable clones can compromise the quality of a BAC library since such "empty" wells in BAC-library plates increases the preparation costs and increases the need for larger number of clones to be processed in fingerprinting if used for physical map construction.
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