Air lock, device that permits passage between regions of differing air pressures, most often used for passage between atmospheric pressure and chambers in which the air is compressed, such as pneumatic caissons and underwater tunnels.
An easement may also be created by implication, when, for example, a term descriptive of an easement is incidentally included in a deed (such as "passageway"—a section of land to be used for passage).
Though it's still possible to stay the night, as Dickens did, I stayed for just a few hours, filling my time with a quick tour of the monastery's oddball museum, which showcased local taxidermy and ancient coins and maps, once used for passage.
The aisles are narrower than in medieval churches and are used for passage rather than seating.
When the cells reached 80 90% confluence of the total area, 2.5 g/l trypsin was used for passage culture.
The Vero cells used for passage and vaccine production were a derivative of a certified cell line manufactured at The Salk Institute, Swiftwater, PA.
Cells were trypsinised (1 mg ml−1 in 0.2% phosphate-buffered saline (PBS)/EDTA), centrifuged (× 2, 400 g, 5 min) and used for passage or further experimental work.
Cells were cultured in DMEM/F12 medium (Invitrogen; 31330-038), 10% serum (Sigma-Aldrich; F7524), 2 mmol/l l-glutamine (Invitrogen; 25030-024), 1% antibiotic/antimycotic (Invitrogen; 15240-062) and 0.5% sodium pyruvate (Invitrogen; 11360-039) and used for passage 4 8.
