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The heterosis level is clearly a function of the combination of the parents used for offspring production.
These results begin to define the genetic mechanisms that may be used for offspring production and development in Epulopiscium.
As the proportion of hybrid females that produced daughters (i.e., were still able to mate and fertilize their eggs) decreased during successive introgression generations [ 21], the number of broods with at least one daughter that could be used for offspring sex ratio analysis decreased gradually.
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The program used for virtual offspring simulation and estimation of pairwise distances within different classes of relatedness is available upon request, as well as the one used for parental allocation.
Seeds were after-ripened for one year in dry conditions before they were used for the offspring germination experiment.
A LMM was used for analyzing average offspring mass, because these data were normally distributed.
This data set is identical to the data set used for the parent-offspring regression, with exception of the use of average values from individuals in the same brood in the latter method.
Most methods used for assigning parents to offspring do not provide a 100% confidence of correct assignment, but usually allow between a 1% and 5% level of error, as was the case in the present study.
Mouse round spermatids the youngest haploid male germ cells appear first at 17 days after birth and can be used for the production of offspring by round spermatid injection (ROSI) into oocytes [9].
Thus, offspring used for phenotyping were a mixed B6x[129xB6] background.
The offspring used for the tumor studies were MMTV-PyMT/S14−/− and MMTV-PyMT/ S14+/+ females.
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