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In most cases, actin 5C mRNA was used for normalization purposes.
U6sRNA expression was used for normalization purposes.
Each microarray contained 4 sequences of the rplp0 gene that were used for normalization purposes [ 23].
Ribosomal protein L32 gene was used for normalization purposes [ 21, 55].
Untreated 2D conditions were used for normalization purposes and assigned a value of one arbitrary unit.
According to Agilent they do not appear to behave consistently enough to be used for normalization purposes.
Similar(44)
Differences in Ct values between tested transcripts and hu signals were used for normalization purpose and based on the MHB medium condition at 2h as a reference.
Starting with equal quantities of total RNA, the housekeeping genes Ppia (peptidylprolyl isomerase A or cyclophilin-A, CYC); glyceraldehyde 3-phosphate dehydrogenase, and 18S rRNA were examined first to determine which to use for normalization purposes.
Histogram rescaling is used afterwards for normalization purpose.
GAPDH was used for normalization.
GAPDH was used for normalization of expression.
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