Sentence examples for used for negative selection from inspiring English sources
Thymidine kinase from herpes simplex virus (HSV-tk) was used for negative selection with gancyclovir, and positive selection from neo was achieved by selecting for G418-resistant colonies.
The best markers within the Saltol QTL region were AP3206, RM8094, and RM3412, the most useful markers flanking the Saltol region were RM1287 and RM10694 (telomeric to Saltol) and RM493 and RM10793 (centromeric to Saltol), while nearby markers that can be used for negative selection are RM490 above Saltol and RM562 and RM7075 below (Supplementary Table 6).
Immobilized recombinant extracellular domain of the epidermal growth factor receptor (EGFR) was used for negative selection.
In experiments requiring high purity NK cells, Thy1.2 beads were additionally used for negative selection according to the Miltenyi's protocol.
The targeting vector also contained a thymidine kinase gene was used for negative selection of clones with random integration of the targeting vector.
After extensive washing, bound phages were eluted by pH shift (0.2 M glycine/HCl, pH 2.2), neutralized with 1 M Tris-HCl (pH 9.1) and used for negative selection as described above.
Recombinant extracellular domain of the epidermal growth factor receptor (EGFR) was used for negative control selection.
In contrast to several studies that used SSEA1 for positive selection and enrichment of early-stage ESC-derived PGCs (11, 13, 17, 51), we used SSEA1 for negative selection to allow the isolation of Oct4-GFP+ and SSEA1− post-PGC-stage germ cells that exhibited properties of meiosis and maturation.
Consistent with its use for negative selection, transgenic Arabidopsis plants were generated with P450 SU1 situated between recognition sequences for FLP recombinase from yeast to select for recombinase-mediated excision.
PBMCs were enriched for CD4+ T cells using MACS beads for negative selection as per manufacturer's recommendations.
We did not test for negative selection using this method.
