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The hearts used for morphological analyses were first injected with potassium chloride (KCl, 100 mM) to ensure each heart was stopped in diastole.
Landmarks used for morphological analyses.
Because of the presence of a distal bone in the rat penis, the midshaft of each organ was used for morphological analyses.
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Some museum samples were used only for morphological analyses, while others from central and eastern Europe were used only to provide context in phylogenetic analyses of molecular characters (Table 1).
Briefly, the analyses were performed on extracted DNA obtained from sections of paraffin blocks, of which the preceding section had been used for morphological diagnosis.
Briefly, analyses were performed on extracted DNA obtained from a 10- μm thick section of paraffin blocks, the preceding section of which had been used for morphological diagnosis.
These individuals were also used for morphological and molecular analysis.
Tissue micro arrays were used for morphological and immunohistochemical comparison.
Standard hematoxylin-eosin staining was used for morphological studies.
These results demonstrate that complementary imaging techniques should ideally be used for comparative morphological analyses, an approach commonly referred to as multimodality.
In total, 295 individuals were measured for morphological analyses.
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