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The LE database contains data points from a total of 976 probe pairs (Note: here the term of "probe" refers to "fluorescent-labeled total RNA sample used for microarray hybridization") that have been hybridized to one or more Human Genome chips 1~5.
RNA samples used for microarray hybridization were obtained from two elite hybrid rice parents MH63 and ZS97 growing in normal field conditions at different developmental stages.
Samples with a label incorporation efficiency of 2 4% were used for microarray hybridization.
Q-PCR analyses were carried out from the same RNA extract used for microarray hybridization.
in vitro transcription proceeded with an incubation time of 4 h at 37° C. 2.5 µg aRNA was used for microarray hybridization.
The RIN of the samples used for microarray hybridization was 8.7±0.3 (mean ± SD) and it was 8.8±0.2 for the samples used in qPCR analysis.
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A reference design was used for microarray hybridizations, such that all RNA samples were labelled using Cy3 and co-hybridized with Cy5 labelled CRR pool.
A reference design was used for microarray hybridizations, such that all RNA samples were labelled using Cy3 and co-hybridized with Cy5 labelled common reference RNA CRRR) pool as described previously [ 24].
These RNA preparations were independent from those used for microarray hybridizations.
The changed expression of these genes was confirmed by qRTPCR in the same RNA samples used for microarray hybridizations (not shown).
In the RNA samples from U251 cells used for microarray hybridizations however, HSF1 expression was induced more than 1.8 folds after NFIX siRNA treatment (nor shown).
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used for microarray validation
used for microarray expression
used for microarray production
used for miRNA hybridization
used for chip hybridization
used for synthesis hybridization
used for microarray analysis
used for microarray gene
used for microarray sample
used for probe hybridization
used for microarray experiment
used for array hybridization
used for membrane hybridization
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