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RNA was extracted and used for microarray gene expression analysis.
Affymetrix U133 Plus 2.0 array was used for microarray gene expression analysis.
RNAs from the 11 cancer cell lines were extracted and used for microarray gene expression analysis.
An aliquot of any given RNA sample used for microarray gene expression analysis was reverse-transcribed to provide the substrate for qRTPCR quantification.
Total RNA was extracted from TM 86 and TM 93 cells treated with 1 μM DEX, FA, or TA for 24 hr and used for microarray gene expression analysis.
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Because the embryos used for microarray analysis of gene expression were offspring from heterozygous parents, we could not exclude the possibility that maternally deposited MyD88 influenced our observations.
In addition, the origin of the libraries in terms of plant organ or developmental stage, used for microarray construction or gene database may also affect the representation level, as may be the case in this tomato-potato comparison.
To understand the molecular mechanisms underlying selective sensitivity of tamoxifen-resistant cancer stem-like cell population to CXCR4 inhibition, MCF7 TAM-R) and MCF7 TAM-Rrands tuMCF7s harvested after the treatment with Cxenograftsonistumours00 were used for microarray analysis of gene expression using Affymetrix U133 arrays (Supplementary Table S1 and S2).
A second kind of model has been engineered by transfecting the ER negative breast cancer Hs578T cell line with a FLAG-tagged and inducible version of ERα, ERβ or ERβ cx, and was used for microarray analysis of gene expression [ 25].
Measurements were performed in the same RNA samples used for microarray-based gene expression profiling.
A detailed description of the steps used for microarray analysis to obtain gene expression levels are given in a previous publication [ 17].
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