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All samples were used for microarray assays after adjustment to a minimum concentration (50 ng/μl).
Next, RNA samples were used for microarray assays.
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RNA samples used for microarray assay were reevaluated by RTPCR and confirmed the expression of cartilage matrix ECM genes (Figure 3B); however, histological cartilage tissue was not formed in the inner chamber of T-shaped implant (Figure 1C).
This discrepancy may be due to the small sample size (n = 43) used for microarray assay [ 16].
RNA samples of more than 2 μg/μl concentration and high purity (OD260/280 > 2, OD260/230 > 2) were used for microarray assay and RT-PCR.
Also, the normalization method used for microarray and QRT-PCR assays was different, especially in the number of genes used for normalization for microarray versus QRT-PCR.
Selected cells were expanded on regular plates for one week before being used for microarray analysis and functional assays.
RNA samples used for qRT-PCR were the same used for microarray experiments, the qRT-PCR assays were designed with Primer Express 2.0 software (Applied Biosystems) to select appropriate primer sequences from known sheep or bovine sequences.
The RNA samples were used for microarray analysis and quantitative real time-PCR (qRT-PCR) assays.
The plants were used for microarray experiments and expression pattern analyses of MtCBF4 under different stress assays.
* used for microarray analysis; ** used to investigate gemcitabine resistance; ° used for sphere-formation assay.
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