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Different Brassica species and wild relatives collected from Centre for Genetic Manipulation of Crop Plants, Delhi University South Campus, India Koreaa Brassica Genome Research Bank, Korea; and Leibniz institute of Plant Genetics and Crop Plant Research, Gatersleben, Germany were used for marker transferability analysis (Table 1).
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Two accessions (H1 and H7) of H. chilense and one genotype ('Barke') of H. vulgare were used for the initial transferability analysis of 82 barley EST-derived SSR-markers.
New developed InDel markers and SNP markers used for mapping.
SSRs are the favoured marker type used for many applications in plant genetics including genetic mapping because of easy transferability between intraspecific populations and across closely related species, and a high number of alleles per locus that provides greater information content [ 45- 48].
In addition, this paper shows that structurally different environments can be used for the two phases without compromising transferability.
Photomarking machines are used for duplicating often-used paper markers.
Using genomic sequences from non-expressed regions may partially account for the low marker transferability across species.
Assay of SSR markers transferability across different cucurbit species also supported the known phylogeny and demonstrated that the bottle gourd SSR markers could be selectively used for watermelon (41% amplification rate), and loofah (20% amplification rate) if necessary, due to their relatively higher cross-species transferability.
Genomic SSRs have been extensively used for mapping, genetic diversity analysis, and plant breeding, but have a lower rate of transferability across species when compared to the EST-SSR markers.
The success in marker transferability for all cucumber SSRs tested was 7.7% (187/2,442).
The utility of wheat DArT markers for rye genotyping is consistent with the previous data on cross transferability of molecular markers between wheat and rye - it was proven that wheat RFLPs [ 3] and SSRs [ 4] can be successfully used for rye genome analyses.
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