Sentence examples for used for marker excision from inspiring English sources

Next, we showed ZFN could be used for marker excision, and the locus can be targeted again by recombinases.

The use of nucleases for marker excision is of strategic importance as the cut-repair site cannot be targeted by the same nuclease.

We made a construct containing eight anthocyanin-related genes (two regulatory genes from maize and six structural genes from Coleus) driven by the endosperm-specific promoters,plus a selectable marker and a gene for marker excision.

Antunes et al. [ 25] found ~15%% efficiency of PB1 in Arabidopsis for marker excision and inheritance of the excision locus.

Finally, we show that heat-inducible I- SceI is also suitable for marker excision, and therefore could serve as an important tool in streamlining this gene stacking platform.

The present work tested each component of the proposed gene stacking method that involves robust SSR systems for directing gene integrations and nucleases for marker excisions.

When it is used for excision of the selective marker, the initial integration of the cassette is carried out by general recombination [ 3].

SpeI and Acc65I were used for excision of the deletion cassette to be used for transformation.

An anterior approach can be used for fragment excision or fixation using mini-fragment screws.

Techniques used for local excision are transanal surgical tumor removal and endoscopic microsurgical tumor ablation.

In addition, DNA extracted from peripheral blood mononuclear cells at the 17 week time point were used for T cell receptor excision circles (TRECs) analysis as a further marker of thymic function [ 40].